PURE-LAMP Procedure for the Diagnosis of Extrapulmonary Tuberculosis: A Case Series.

نویسندگان

  • Yuji Kouzaki
  • Kei Mikita
  • Takuya Maeda
  • Masashi Ishihara
  • Shuichi Kawano
  • Yuji Fujikura
  • Kazuo Imai
  • Soichiro Kanoh
  • Kazuho Utsunomiya
  • Masahiro Inoue
  • Yasushi Miyahira
  • Akihiko Kawana
چکیده

Although the polymerase chain reaction is effective for the diagnosis of extrapulmonary tuberculosis (EPTB), it is typically unavailable in resource-limited situations. In contrast, the loop-mediated isothermal amplification (LAMP) assay is a relatively cost-effective and accessible method. Additionally, when combined with the procedure for ultra-rapid extraction (PURE) kit, which enables simple DNA extraction, LAMP can detect Mycobacterium tuberculosis in sputum within 1.5 hours using a simple procedure. In this study, we investigated the utility of the PURE-LAMP technique to diagnose three cases of EPTB and showed that it may potentially be a valuable tool for the diagnosis of EPTB.

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عنوان ژورنال:
  • Internal medicine

دوره 54 11  شماره 

صفحات  -

تاریخ انتشار 2015